MicroFlow PLUS Kit

MicroFlow^PLUS Kit (in vivo)

The Litron Advantage is delivered in every kit:

Faster Results	Scoring samples using your own in-house flow cytometer can offer you even more flexibility and power. Typically, a complete Micronucleus Test can be scored in just one day. This is many times faster than the three to six weeks necessary for traditional microscopic methods.
More Data	With the use of a flow cytometer, the MicroFlow process allows a researcher to score samples within a population of 20,000 reticulocytes, versus only 2,000 using traditional microscopic methods. This not only provides much tighter standard deviations, but also more objective data.
Greater Confidence	MicroFlow data is accepted by the US FDA for the mouse micronucleus assay, a test required by regulatory agencies. Additionally, this method addresses all recommendations made by the International Workshop on Genotoxicity Test Procedures (IWGTP). Additionally, the most current Organization for Economic Co-Operation and Development (OECD) guidelines regarding micronucleus testing, Guideline 474, indicates that flow cytometry is an acceptable alternative to microscopic evaluation. Litron developed this patented process and routinely works with researchers using MicroFlow^PLUS kits, so you can feel confident using this proven method in your own lab.

In Vivo MicroFlow kits contain all of the quality-assured supplies and reagents for researchers to collect and fix samples. In the case of the MicroFlow^PLUS kits, additional materials, reagents and biostandards are provided, giving researchers the ability to run their samples in-house. Each kit is available for use with a wide range of species, from mouse and rat up to human. Here's what you get, what it does, and how your research process is improved using MicroFlow^PLUSkits:

Feature	Function	Benefit
Fixed Malaria Biostandards	Contain DNA in the same size range as micronuclei. It is used for setting up and calibrating the flow cytometer	Properly calibrates the flow cytometer so that only micronucleus events are scored. Reduces day-to-day staining variability.
Solution A (fixative)	Fixes blood cells prior to evaluation by flow cytometry.	Cells can be maintained for months or years at -85 °C. This gives researchers the ability to accumulate samples over time, then analyze multiple time points at once.
Solution B (anticoagulant)	Prevents blood cells from clotting before fixation.	Allows samples to be fixed up to 6 hours after collection.
Solution C (buffer)	Washes fixed cells out of Solution A, and acts as a base for stocks D, E, F and G.	Prevents excessive cell lysis and ensure compatibility with other reagents.
Solution D (RNase)	Degrades cellular RNA.	Allows Solution F to be DNA-specific.
Solution E (antibody)	Labels the youngest red blood cells (reticulocytes) by binding to a receptor on the outer surface of the cell.	Specifically labels the population of interest for MN scoring, and allows differentiation from mature red blood cells.
Solution F (stain)	Stains DNA.	Combins with Solution D to achieve DNA-specific staining of micronuclei and Biostandards.
Solution G (anti platelet antibody)	Fluorescently labels platelets.	Allows users to "gate out" platelets, which could otherwise be mistaken for micronucleus counts.