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MicroFlowPLUS Kit (in vivo)
The Litron Advantage
is delivered in every kit:
| Faster
Results |
Scoring samples using your own in-house flow cytometer can offer
you even more flexibility and power. Typically, a complete
Micronucleus Test can be scored in just one day. This is many times faster than the three to six weeks
necessary for traditional microscopic methods. |
| More
Data |
With the use of a flow cytometer, the MicroFlow process allows
a researcher to score samples within a population of 20,000
reticulocytes, versus only 2,000 using traditional microscopic
methods. This not only provides much tighter standard deviations,
but also more objective data. |
| Greater
Confidence |
MicroFlow data is accepted by the US FDA for the mouse
micronucleus assay, a test required by regulatory
agencies. Additionally, this method addresses all
recommendations made by the International Workshop on
Genotoxicity Test Procedures (IWGTP), and the most
current Organization for Economic Co-Operation and
Development (OECD) guidelines regarding micronucleus
testing, Guideline 474, indicates that flow cytometry is
an acceptable alternative to microscopic evaluation.
Litron developed this patented process and routinely
analyzes samples from MicroFlowBASIC kits, so
you can feel confident using this proven method. |
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In Vivo MicroFlow
kits contain all of the quality-assured supplies and reagents for
researchers to collect and fix samples. In the case of the MicroFlowPLUS
kits, additional materials, reagents and biostandards are provided,
giving researchers the ability to run their samples in-house. Each
kit is available for use with a wide range of species. Here's what
you get, what it does, and how your research process is improved
using MicroFlowPLUS kits:
| Feature |
Function |
Benefit |
| Fixed
Malaria Biostandards |
Contain DNA in the same size range as micronuclei. It is used for setting
up and calibrating the flow cytometer |
Properly calibrates the flow cytometer so that only micronucleus
events are scored. Reduces day-to-day staining variability. |
| Anticoagulant/Diluent |
Prevents blood cells from clotting before fixation. |
Allows samples to be fixed up to 6 hours after collection. |
|
Buffer
Solution |
Washes cells
out of fixative, and acts as a base for other solutions. |
Prevents excessive cell lysis and ensure compatibility with other
reagents. |
|
Long Term Storage Solution (LTSS) |
Provides
increased stability of samples. |
Allows
sample integrity to be maintained during storage and
shipping even in the case of temperature
fluctuations. Eliminates the need for ultracold methanol
and dry ice during shipping. |
| RNase
Solution |
Degrades cellular RNA. |
Allows stain
to be DNA-specific. |
| Anti-CD71 Antibody |
Labels the youngest red blood cells (reticulocytes) by binding
to a receptor on the outer surface of the cell. |
Specifically labels the population of interest for MN scoring, and allows differentiation from mature red blood cells. |
|
DNA Stain |
Stains DNA. |
Combines
with RNase to achieve DNA-specific staining of
micronuclei and Biostandards. |
| Platelet
Antibody |
Fluorescently
labels platelets. |
Allows users
to "gate out" platelets, which could otherwise be mistaken for micronucleus counts. |
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