Measure DNA damage at the Pig-a locus
|
|
This test detects mutations at the Pig-a gene that cause failure of “GPI anchors”. Without these anchors, specific markers on the exterior of cells will not be present. This assay measures the frequency of cells without these surface markers, also known as “Pig-a mutant cells”.
|
Prototype MutaFlow® Kits
|
|
|
Litron's MutaFlow kits use flow cytometry to measure the frequency of Pig-a mutant red blood cells in vivo.
Below, the wild type cell on the left expresses CD59. The cell on the right has a mutation in the Pig-a gene and therefore does not express CD59 on the cell surface. This difference is easily detected with a flow cytometer.
|
Click Image to Enlarge
|
Problems with existing methods
|
| |
Expensive transgenic animals and labor-intensive cell culture work have prevented in vivo mutation assays from integrating into other studies. MutaFlow Kits solve these issues because they do not require transgenic animals or cell culture work. |
Main Benefits
- Easily integrates into existing studies
- No expensive transgenic animals required
- Fast results (analysis by flow cytometry)
Other Advantages
- Requires very low volumes of blood
- Does not involve cell culture work
- Reduces animal use
Validation study
|
|
|
A multi-laboratory Pig-a trial
is currently under way. This work is being performed by industry, government and
regulatory organizations from around the world. For more information, see
these journal articles or
contact us. |
|
