Measure DNA Damage in Red Blood Cells
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This test detects damage to the chromosomes, or spindle
apparatus, of cells. After exposure to a test substance, precursor cells divide. It is upon division that this type of damage can result in the formation of a smaller ‘micronucleus', apart from the main nucleus.
At this point, red blood cells expel their nuclei. When stained, micronuclei (i.e., pieces of DNA) are
easily seen in a population of cells with no nuclear DNA.
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MicroFlow® Kits
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Litron's MicroFlow kits use flow cytometry to analyze micronuclei in vivo.
Anti-CD71 antibodies distinguish mature and immature RBCs.
Micronuclei are easily identified with a DNA stain. Biological standards supplied with the kits
allow reproducible results: day-to-day and lab-to-lab.
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Click Image to Enlarge
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New
ICH Guidelines
for In Vivo Micronucleus
Assays
- Laboratories scoring 2,000 cells per sample may now need to score several times as many.
- Rat blood now accepted, allowing for integration into General Tox studies.
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Benefits
- Conforms to New ICH Guidelines (20,000 RETs scored per sample)
- Provides analysis of entire study in one day
- Supplies Mode of Action information
(aneugens versus clastogens)
- Uses biological standards to get reproducible results, across days and between labs
Unlimited Technical Support
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Get answers to your questions, help with validation studies, or send example plots for troubleshooting. We're always here to assist you.
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Bone Marrow or Blood
- Mouse
- Rat
- Other species available
Advantages of Peripheral Blood
- Easily integrates into existing general toxicology studies
- Integration reduces the number of animals in your studies
- Allows each subject to be sampled multiple times
- Requires very low volumes of blood
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