In Vitro Micronucleus

Measure DNA Damage in Cell Cultures

This test detects damage to the chromosomes, or spindle apparatus, of cells. After exposure to a test substance, cells are allowed to divide. It is upon division that this type of damage can result in the formation of a smaller ‘micronucleus', apart from the main nucleus.

MicroFlow® Kits

Litron's MicroFlow kits use flow cytometry to analyze micronuclei in vitro.

The key element of this method is the sequential staining. One staining step identifies dead and dying cells. The second step lyses cells and stains DNA. This approach allows you to distinguish micronuclei from other events, such as apoptotic bodies.

In Vitro Micronucleus; In Vitro MicroFlow Kit
Click Image to Enlarge

New ICH Guidelines
for In Vitro Micronucleus Assays

Now accepted for GLP submissions.


Hundreds of samples analyzed in a few hours. Eliminates tech time with "walk away" analysis using auto samplers.

High Content

Obtain these additional endpoints:

  • Relative survival
  • Membrane integrity
  • Cell cycle
  • Mode of Action
  • Low dose threshold (NOEL)

Unlimited Technical Support

Get answers to your questions, help with validation studies, or send example plots for troubleshooting. We're always here to assist you.

Cell Lines

Use a variety of attachment and suspension cell lines, including:

  • L5178Y
  • CHO-K1
  • TK6
  • V79
  • WIL-2
  • HepG2

Skin models and other types of cells and tissues are in development.  |  585-442-0930 (USA)