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Litron is pleased to announce the launch of its In Vitro MicroFlowTM Kits.
Requiring only minimal amounts of compound, this method supports both attachment and suspension cell lines and
significantly improves the speed and objectivity of scoring in vitro Micronucleus samples.
Two In Vitro MicroFlow kits are available:
• 100 sample (trial version)
• 500 sample
Litron Highlighted on Cover of E&MM Journal
The cover of this month's edition of Environmental and Molecular Mutagenesis features
Litron's novel Pig-a mutation assay. Supporting all mammalian species, this method
is described in the article "In Vivo Mutation Assay Based
on the Endogenous Pig-a Locus".
[more info] |
In Vitro Micronucleus Analysis
The Next Evolution of MicroFlowTM Kits
Compatible with attachment and suspension cell lines
including L5178Y, CHO-K1, TK6 and V79.
Multiple Endpoints:
High Content Analysis
In addition to the micronucleus endpoint, researchers can obtain simultaneous
information about cell cycle effects, apoptosis/necrosis,
and cytotoxicity. By scoring more than 10,000 cells per sample,
this method provides higher scoring precision
compared to microscopic methods.
Protects against false positives
Based in part on the high
information content described above, data may be used to make more informed decisions
regarding top concentration. This helps protect against false positives, as described in the
graphs below.
Application
of the appropriate cytotoxicity endpoint is essential for proper selection
of top drug concentration. Both staurosporine and dexamethasone, classified
as non-genotoxic cytotoxicants, show a "false-positive" response by flow cytometry
if relative survival is determined by Coulter Counter. The use of In Vitro MicroFlow's
assessment (Nuclei-to-Bead Ratio) reveals less than 40 % survival at the two highest concentrations,
thus eliminating these concentrations from consideration.
Enables you to keep up with quickly growing demand
Each measurement is accomplished in less than two minutes.
Minimal compound required
Use of In Vitro MicroFlow kits during early toxicity screening provides the perfect complement
to the FDA required in-life testing covered by our In Vivo MicroFlow technology.
REACH
(Registration, Evaluation,
Authorisation, and Restriction of Chemicals)
New European legislation, known as REACH, has been enacted, requiring companies to prove the safety of
the chemicals they produce. Based on this law, approximately 30,000 chemicals will need to be registered
over eleven years. MicroFlow kits provide a superior method to achieve the micronucleus assessments
set forth in the REACH initiative.
Please contact
Litron for more information. |
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See Us At These Meetings
Scientists from Litron have recently been invited to
present our flow cytometric methods at:
- ILSI Health and Environmental Sciences
Institute, Project Committee On the
Relevance and Follow-Up of Positive Results in In Vitro Genetic Toxicology
(IVGT) Testing, International Workshop, Washington, DC, May 21 - 22, 2008.
- ECVAM Workshop, Ispra, Italy, June 24 - 25, 2008
- EEMS 8th
Annual Meeting
"Environmental Mutagens and Human Health", Cavtat, Croatia, September 21 - 25, 2008.
"Flow cytometric scoring of micronucleated
reticulocytes: bridging species of toxicological interest".
- EMS 39th
Annual Meeting, Rio Grande, Puerto Rico,
October 18 - 22, 2008.
- JEMS 37th
Annual Meeting, Communication - People,
Environment and Risk, Okinawa, Japan, December 4 - 6, 2008.
In addition to these meetings, we are providing training on our in vivo and
in vitro methods to scientists throughout the world
(most recently in the UK, Germany and USA). |
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