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eNewsletter, Summer 2009
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Summer 2009
Innovative Pig-a mutation assay Get an update at
UKEMS
There is considerable interest
throughout the scientific community about the in vivo
mutation assay based on the endogenous pig-a gene..
Dr. Stephen Dertinger will present the latest research
on this method at the 2009 United Kingdom Environmental
Mutagen Society in his talk entitled: "Cross-species
mutation assay based on the Pig-a gene". [more
info]
Pig-a Workshop at EMS
Dr. Stephen Dertinger (Litron)
and Dr. Robert Heflich (FDA-NCTR) will co-chair an exciting
workshop where experts in the field will present their
latest research. Presentations will include: assay background
and methodology, rodent mutation data, kinetics, DNA
sequencing results as well as a discussion relative
to oncology. The workshop is scheduled for the Saturday
afternoon before the St. Louis EMS meeting, and a separate
registration is required. [more
info]
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User SpotLite
BioReliance Adopts MicroFlow®
Technology
Micronucleus Analysis
by Flow Cytometry
BioReliance, one of the world's leading contract research
organizations, has completed internal validation of
both the In Vitro and In Vivo MicroFlow
methods. With this work successfully finished, the company
is now providing MicroFlow analyses to its clients as
part of its standard battery of genetic toxicology tests.
Robert Young, Global Toxicology Technical Specialist
at BioReliance, outlined several key reasons his company
decided to offer MicroFlow-based analyses:
- The MicroFlow technology provides high information
content. In particular, we're excited about the
MicroFlow kits' ability to provide information on
clastogenic versus aneugenic modes of action. BioReliance
scientists presented supporting data at recent meetings
and were received with enthusiasm.
- MicroFlow protocols are applicable across species
and across cell lines. This flexibility is very
important to BioReliance, as it allows us to more
easily address each of our client's unique needs.
Also, the kits' flow cytometric platform allows
us to efficiently multiplex different endpoints.
For instance, micronucleus and Comet assays can
be performed in parallel in both in vitro
studies and integrated into the same in vivo
toxicity study.
- Litron personnel go out of their way to make
the transition to automated scoring an easy one.
The level of technical support that Litron provides
is exceptional.
Dr. Stephen Dertinger, Director of Research at Litron,
expressed his admiration for the quality of the work
conducted by the BioReliance staff. "We were impressed
with the way they accomplished their validation work,
so carefully and systematically. It was really great
to see them efficiently execute these studies and generate
such compelling data. I hope they decide to publish
their results, because their approach truly represents
a wonderful blueprint for others who wish to internally
validate a new scoring method."
Incorporating this technology into its already impressive
suite of preclinical toxicology services, BioReliance
is well-positioned to provide superior service to its
clients for many years to come.
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See Litron's Technologies Presented at these
Meetings
UKEMS
Leeds, UK July 12-15, 2009
more info
- "Cross-species
mutation assay based on the Pig-a gene",
presentation by
Litron Laboratories
- "Assessment
of intra-individual variation in micronucleated
reticulocytes in humans using flow cytometry
and comparison with chromosome aberration
data. A potential biomarker for human genotoxicity?",
poster by
GlaxoSmithKline
ICEM
Florence, Italy August 20-25, 2009
more info
- "Cross-Species,
Endogenous Mutation Assay Based on the
Pig-a
Gene", presentation by
Litron Laboratories
- "Further evaluation
of a flow cytometric in vitro micronucleus
assay in CHO-K1 cells: a reliable platform
that detects micronucleus and discriminates
apoptotic bodies", poster by
BioReliance
- "Flow cytometric
analysis of micronuclei in rat peripheral
blood: An Interlaboratory Evaluation", poster
by
Biosafety Research Center
- "Assessment
of intra-individual variation in micronucleated
reticulocytes in humans using flow cytometry
and comparison with chromosome aberration
data. A potential biomarker for human genotoxicity?",
poster by
GlaxoSmithKline
- "Response kinetics
if Pig-a and LacZ mutations, and micronucleus
induction by benzo(a)pyrene in Muta™Mouse
hematopoietic cells", poster by
Health Canada
- "In Vitro
Micronucleus Scoring by Flow Cytometry:
A High Throughput Approach Providing Simultaneous
Mode of Action", poster by
Litron Laboratories
- "Pig-a Mutation
and Micronucleus Scoring by Flow Cytometry:
Integration into Acute Dosing Schedule",
poster by
Litron Laboratories
GTA (Abstracts submitted)
Newark, DE September 9-10, 2009
more info
- "Incorporating
flow cytometry based automated scoring into
the reconstructed skin micronucleus assay",
poster by
Institute for In Vitro Sciences
- "In vivo
Rat Pig-a Mutation Assessment by Flow Cytometry:
Inter-Laboratory Comparison of ENU Treated
Rats", poster by
Pfizer
Eurotox
Dresden, Germany September 13-16, 2009
more info
- "Multi-lab
validation of an improved flow cytometric
in vitro micronucleus assay using
attached cell lines", poster by
BioReliance
- "In Vitro
Micronucleus Testing by Flow Cytometry:
Greater Throughput, High Information Content",
poster by
Litron Laboratories
- "Micronucleus
and Mutation Assessment by Flow Cytometry:
Integration into Rodent Repeat-Dose Studies",
poster by
Litron Laboratories
EMS
St. Louis, MO October 24-28, 2009
more info
- "In Vitro
Micronucleus Screening of Pharmaceutical
Candidtates by Flow Cytometry in Chinese
Hamster V79 Cells", poster by
Abbott
- "Flow Cytometric
In Vitro Micronucleus Scoring Provides
Simultaneous Mode of Action Information",
poster by
Litron Laboratories
- "Pig-a Mutation
and Micronucleus Scoring by Flow Cytometry:
Integration into an Acute Dosing Schedule",
poster by
Litron Laboratories
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"In vivo Rat Pig-a Mutation Assessment
by Flow Cytometry: Inter-Laboratory Comparison
of ENU Treated Rats", poster by
Pfizer
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