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Research and Development
In Vitro Micronucleus
Development of the in vitro MicroFlow method has progressed through Beta-testing and two new kits have recently been released. Further optimization of this process, including the incorporation of automated liquid handling and a miniaturized format for high throughput analyses, continues.
Human Micronucleus (Howell-Jolly Bodies)
In hematology, the micronucleus inclusion found in a red blood cell is called a Howell-Jolly Body (HJB). An increased frequency of HJBs in human blood can indicate numerous physiological conditions including loss of splenic function or damage to red blood cell precursors.
Rat Micronucleus (ICH Validation)
The majority of in vivo studies conducted by pharmaceutical or chemical companies that are designed to look specifically at genetic damage are performed in mice. Other studies that examine more general, long-term toxicity of drugs and chemicals are performed in rats. An extensive collaboration between government agencies, pharmaceutical companies, contract research organizations and Litron Laboratories has begun that seeks to validate In Vivo MicroFlow in the rat as an effective method to examine genotoxic mode of action.
Endogenous Mutation Assay
Current mutation assays are burdened by issues such as time-consuming, labor-intensive cell culture techniques or the requirement for prohibitively expensive transgenic animal models. The development of an assay system that takes advantage of an easily detected endogenous mutation locus could provide simple, rapid assessment of a chemical’s mutagenic potential. One of the primary benefits of this system would include the ability to examine a mutation-based endpoint in cells in culture as well as in many species of toxicological interest.
Bone Marrow Micronucleus
Traditionally the in vivo micronucleus test was performed on cells derived from the bone marrow of treated animals. The in vivo MicroFlow method has now been adapted to examine cells that originate from bone marrow. This allows for the direct comparison of micronucleus formation in both blood and bone compartments as a means for comparison to historical controls and further validation of observations made in peripheral blood.
Radiation Damage Detection
In addition to chemical genotoxicants, radiation exposure can result in the induction of significant chromosome damage. Following the ever-increasing interest in response to accidental or intentional exposure of human populations to radiation, the development of new and effective methods to detect radiation damage in humans is critical. Litron Laboratories is taking part in a large collaborative effort between academia and government funding agencies to develop new and innovative techniques to monitor the effects of radiation in humans.
Collaboration Opportunities
Litron Laboratories has established numerous collaborations with groups in industry, academia and government agencies. These interactions continue to be of great value for all parties involved. We see our work with the experts in the field as an integral part of our efforts to improve the way researchers approach genetic toxicology.
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