The process of staining and lysis is quite simple.  Following treatment, the cells are carried through a staining scheme that first labels dead and dying cells and then simultaneously lyses and labels nuclear material.  From the start of procedure, to when the samples are ready for flow cytometric analysis, the process takes about 3.5 hours for 50 samples.  It should be noted that a majority of this time is taken up by incubation steps where the operator is free to perform other tasks.

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